engleski

Partial Hepatectomy and Diets Enriched with Olive and Corn Oil Altered the Phospholipid Fatty Acid Profile in the Spleen

The purpose of this study was to examine changes in the polar fatty acid (PL FAs) profile in mice spleen after a one-third partial hepatectomy (PHx) and a diet enriched with olive and corn oil. Fatty acids (FAs) were determined by gas chromatography (GC) after previous fractionation of polar fatty acids by solid-phase extraction using an aminopropyl (NH2) column. The data were analysed using the nonparametric Kruskal-Wallis test and linear regression analysis. A diet supplemented with corn oil (FCO) increased palmitic acid, while an olive oil-enriched diet (FOO) increased arachidonic and docosahexaenoic acid in the spleen PL FAs during PHx. Based on the FAs profile of PL FAs in the spleen during PHx, in the FCO diet group stearoyl CoA desaturase (SCD1) activity showed a positive correlation (R=0.58) with 18:2n-6 as the major FAs in corn oil, while in the FOO group, SCD1 and elongase- 6 (Elovl6) activities positively correlated (R=0.84, R=0.55, respectively) with 18:1n-9 as the major FAs in olive oil. To conclude, despite the beneficial effect of diet, lipid homeostasis in the spleen was regulated more by PHx than the n-6 and n-9 diet.

Fatty acids ; Phospholipids ; Corn oil diet ; Olive oil diet ; Partial hepatectomy

This study was supported by the Croatian Ministry of Science, Projects No. 0062002, 062-0000000-0221 and 062-0621341-0061. None of the authors has conflicts of interest with respect to this work.