engleski

Extracellular metabolites of selected LAB strains decrease TNF-alpha in LPS stimulated peripheral blood leukocytes

Beneficial health effects of lactic acid bacteria (LAB) used in functional foods and pharmaceutical products are based on the ability and capacity of these microorganisms in stimulation of host immune system. Health improving immune modulation and production of bioactive substances are restricted only to some LAB strains. In this study, peripheral blood mononuclear cells (PBMC) were stimulated with bacterial lipopolysaccharide (LPS) to produce pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α). The aim of this study was to determine in vitro if extracellular bacterial metabolites, with molecular mass smaller than 2000 Da, from four selected LAB strains, suppress TNF-α production in LPS stimulated PBMC and therefore exhibit anti-inflammatory effect. Furthermore, cytotoxicity and genotoxicity of used bacterial culture media fractions containing these metabolites on PBMC was determined. Bacterial fractions showed no cytotoxicity or genotoxicity on PBMC, and all selected strains showed significant suppression of TNF-α production in LPS stimulated cells.

LAB ; probiotic ; LPS ; TNF, metabolites

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