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Epitope mapping of a macrozone interaction with ribosome by NMR spectroscopy and molecular modeling (CROSBI ID 688604)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Mikulandra, Ivana ; Škevin, Sonja ; Lulić, Ana- Marija ; Habinovec, Iva ; Jednačak, Tomislav ; Bertoša, Branimir ; Novak, Predrag Epitope mapping of a macrozone interaction with ribosome by NMR spectroscopy and molecular modeling // Adriatic NMR Conference 2019 : Book of abstracts / Namjesnik, Danijel ; Novak, Predrag ; Parlov Vuković, Jelena (ur.). Zagreb, 2019. str. 57-57

Podaci o odgovornosti

Mikulandra, Ivana ; Škevin, Sonja ; Lulić, Ana- Marija ; Habinovec, Iva ; Jednačak, Tomislav ; Bertoša, Branimir ; Novak, Predrag

engleski

Epitope mapping of a macrozone interaction with ribosome by NMR spectroscopy and molecular modeling

Macrolide antibiotics, such as azithromycin have been widely used to treat infections of both gram- positive and gram-negative bacteria. They exert their biological activity by binding to bacterial ribosome at or near the peptidyl transferase center thus inhibiting protein biosynthesis. Emerging multi-drug resistant microbial pathogens present today a serious and challenging problem in medical treatment which demands novel antimicrobial agents to be obtained. In order to discover and design more effective macrolide compounds, it is important to explore the interactions with ribosome and other biological targets and to elucidate and characterize bound conformations in solution. We have prepared a series of bioactive azithromycin conjugates, the macrozones, by coupling thiosemicarbazones to azithromycin. Here we present results of interaction studies of a selected macrozone and ribosome isolated from E. Coli. We used a combination of NMR experiments such as transferred nuclear Overhauser effect spectroscopy (trNOESY) and saturation transfer difference (STD) and molecular modeling to characterize binding epitopes and asses bound conformations.

macrolide antibiotics, conformations, epitope mapping, STD, trNOESY

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Podaci o prilogu

57-57.

2019.

objavljeno

Podaci o matičnoj publikaciji

Adriatic NMR Conference 2019 : Book of abstracts

Namjesnik, Danijel ; Novak, Predrag ; Parlov Vuković, Jelena

Zagreb:

978-953-6076-56-7

Podaci o skupu

Adriatic NMR 2019

poster

06.06.2019-09.06.2019

Mali Ston, Hrvatska

Povezanost rada

Kemija