Epitope mapping of a macrozone interaction with ribosome by NMR spectroscopy and molecular modeling (CROSBI ID 688604)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Mikulandra, Ivana ; Škevin, Sonja ; Lulić, Ana- Marija ; Habinovec, Iva ; Jednačak, Tomislav ; Bertoša, Branimir ; Novak, Predrag
engleski
Epitope mapping of a macrozone interaction with ribosome by NMR spectroscopy and molecular modeling
Macrolide antibiotics, such as azithromycin have been widely used to treat infections of both gram- positive and gram-negative bacteria. They exert their biological activity by binding to bacterial ribosome at or near the peptidyl transferase center thus inhibiting protein biosynthesis. Emerging multi-drug resistant microbial pathogens present today a serious and challenging problem in medical treatment which demands novel antimicrobial agents to be obtained. In order to discover and design more effective macrolide compounds, it is important to explore the interactions with ribosome and other biological targets and to elucidate and characterize bound conformations in solution. We have prepared a series of bioactive azithromycin conjugates, the macrozones, by coupling thiosemicarbazones to azithromycin. Here we present results of interaction studies of a selected macrozone and ribosome isolated from E. Coli. We used a combination of NMR experiments such as transferred nuclear Overhauser effect spectroscopy (trNOESY) and saturation transfer difference (STD) and molecular modeling to characterize binding epitopes and asses bound conformations.
macrolide antibiotics, conformations, epitope mapping, STD, trNOESY
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Podaci o prilogu
57-57.
2019.
objavljeno
Podaci o matičnoj publikaciji
Adriatic NMR Conference 2019 : Book of abstracts
Namjesnik, Danijel ; Novak, Predrag ; Parlov Vuković, Jelena
Zagreb:
978-953-6076-56-7
Podaci o skupu
Adriatic NMR 2019
poster
06.06.2019-09.06.2019
Mali Ston, Hrvatska