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Adhesion and Aggregation Ability of Probiotic Strain Lactobacillus acidophilus M92

Aims: To investigate aggregation and adhesion abilities of Lact. acidophilus M92 to porcine ileal epithelial cells in vitro, and the influence of cell surface proteins on autoaggregation and adhesiveness of this strain. Methods and Results: Lact. acidophilus M92 exhibits a strong autoaggregating phenotype and manifests a high degree of hydrophobicity determined by microbial adhesion to xylene. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase and pepsin. SDS-PAGE of cell surface proteins revealed the presence of potential surface layer (S-layer) proteins, approximated at 45 kDa, in Lact. acidophilus M92. The relationship between autoaggregation and adhesion ability to intestinal tissue was investigated by observing the adhesivity of Lact. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 mol l-1 LiCl reduced autoaggregation and in vitro adhesion of this strain. Conclusions: These results demonstrate that there is relationship between autoaggregation and adhesion ability of Lact. acidophilus M92, mediated by proteinaceous components on the cell surface. Significance and Impact of the Study: This investigation has shown that Lact. acidophilus M92 has the ability to establish in the human gastrointestinal tract, which is an important determinant in the choice of probiotic strains.

adhesion; aggregation; hydrophobicity; Lactobacillus acidophilus; probiotic properties; surface layer proteins

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