Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Peripheral binding of ethopropazine to horse serum butyrylcholinesterase (CROSBI ID 492845)

Prilog sa skupa u zborniku | izvorni znanstveni rad | međunarodna recenzija

Reiner, Elsa ; Šinko, Goran ; Štuglin, Anita ; Simeon-Rudolf, Vera Peripheral binding of ethopropazine to horse serum butyrylcholinesterase // Cholinergic mechanisms : Function and Dysfunction : Proceedings of the XIth ISCM / Fisher, Abraham ; Silman, Israel ; Soreq, Hermona et al. (ur.). London : Delhi: Francis & Taylor, 2004. str. 673-674-x

Podaci o odgovornosti

Reiner, Elsa ; Šinko, Goran ; Štuglin, Anita ; Simeon-Rudolf, Vera

engleski

Peripheral binding of ethopropazine to horse serum butyrylcholinesterase

The inhibition of purified horse serum butyrylcholinesterase (BChE) with ethopropazine (0.25-20 uM) was studied in order to evaluate the binding site(s) on the enzyme. Activities were measured spectrophotometrically with acetylthiocholine (ATCh ; 0.05-80 mM) as substrate at 37º C in 0.1 M phosphate buffer pH=7.4. The pS-curve for the ATCh hydrolysis fitted well the Webb equation: Ks and Kss = 0.25 and 2.0 mM respectively, beta=3.2. This equation assumes two binding sites for the substrate on the enzyme, catalytic and peripheral, and the beta-value above unity indicates apparent substrate activation. Inhibition of BChE with ethopropazine was non-competitive at substrate concentrations up to 1.0 mM. The enzyme-inhibitor dissociation constant was 0.81 uM. Competition between ATCh and ethopropazine occured at substrate concentrations above the Kss value for ATCh. Such inhibition pattern indicates binding of ethopropazine to the peripheral, non-productive, site on the enzyme.

butyrylcholinesterase; ethopropazine; mechanism; inhibition

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

673-674-x.

2004.

objavljeno

Podaci o matičnoj publikaciji

Cholinergic mechanisms : Function and Dysfunction : Proceedings of the XIth ISCM

Fisher, Abraham ; Silman, Israel ; Soreq, Hermona ; Anglister, Lili ; Michaelson, Daniel M.

London : Delhi: Francis & Taylor

1841840750

Podaci o skupu

Nepoznat skup

poster

29.02.1904-29.02.2096

Povezanost rada

Kemija