engleski

The effect of protein growth factor on the proliferation of continuous cell lines and primary cell cultures

Cell culture systems offer possibilities to screen effects of compounds like hormones, growth factors and drugs in controlled way and under a wide variety of environmental conditions. Proliferation of animal cells in a culture is under control of exogenous growth factors that are mostly present in a serum. Some of the growth factors are specific for particular type of cells, while others are less specific and have effects on many cell types. Recently those polypeptide molecules are also recognised as physiological regulators and now are considered to play a substantial role in the development, tissue remodelling, tissue repair and response to injury, haematopoiesis and tumorigenesis. It was demonstrated that the serum could be reduced or omitted without apparent cellular adaptation if appropriate nutritional and hormonal modifications are made to the medium, so nowadays biotechnology is more and more based on use of the serum-free medium. In our recent work, we have found out that the addition of the protein growth factor (PGF), isolated from swine brain, obtained by our own laboratory, can significantly reduce a need for serum during the cultivation of the cell line BHK21 C13 and at the same time enhance a cell proliferation. Therefore the aim of this study was to determine influence of the PGF on the cell proliferation of the cell line CHO K1 and primary cell culture from the rat's ovary.

ovarian primary cell culture; protein growth factor; proliferation

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