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Effect of Bgl2p and Ccw12p Mannoproteins on Stability of Yeast Cell Wall (CROSBI ID 467865)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Teparić, Renata ; Barbarić, Slobodan ; Mrša, Vladimir Effect of Bgl2p and Ccw12p Mannoproteins on Stability of Yeast Cell Wall // 3. hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir ; Colić-Barić, Irena ; Glancer-Šoljan, Margareta (ur.). Zagreb: Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 1998. str. 148-148-x

Podaci o odgovornosti

Teparić, Renata ; Barbarić, Slobodan ; Mrša, Vladimir

engleski

Effect of Bgl2p and Ccw12p Mannoproteins on Stability of Yeast Cell Wall

The cell wall of the yeast S. cerevisiae contains a number of mannoproteins and for most of them physiological function is unknown. Mannoproteins are either loosely bound to the wall and can be extracted by SDS, or strongly bound and can be extracted only upon glucanase treatment, suggesting covalent bounding to glucan in the cell wall. Most abundant detergent-extractable protein is Bgl2p protein, while Ccw12p is major protein among those strongly bound to the wall. Bgl2p protein shows endoglucanase and glucosyltransferase activity in vitro, indicating a role of this protein in the cell wall glucan assembly, while Ccw12p protein is not characterized yet. In order to assess possible physiological role of those proteins, mutant strains are constructed by disruption of BGL2 or CCW12 genes. Sensitivity of the cell wall to beta-1, 3-glucanase treatment was examined and both mutant strains had increased sensitivity in comparison to the parental strains, showing that these proteins contribute somehow to cell wall stability. However, transformation of mutant strains with episomal plasmid containing CCW12, results in reversion of wild type level of glucanase sensitivity in ccw12, but not in the bgl2 strain. This shows that two proteins affect cell wall stability by different mechanisms. In addition, it was found that overexpression of CCW12 in the wild type cells did not cause increased stability of the cell wall.

S. cerevisiae cell wall; yeast mannoproteins

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Podaci o prilogu

148-148-x.

1998.

objavljeno

Podaci o matičnoj publikaciji

3. hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista

Marić, Vladimir ; Colić-Barić, Irena ; Glancer-Šoljan, Margareta

Zagreb: Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu

Podaci o skupu

3. HRVATSKI KONGRES PREHRAMBENIH TEHNOLOGA, BIOTEHNOLOGA I NUTRICIONISTA

poster

10.06.1998-12.06.1998

Zagreb, Hrvatska

Povezanost rada

Prehrambena tehnologija