Genotypic and phenotypic diversity of lactic acid bacteria isolated from controlled sauerkraut fermentation (CROSBI ID 509315)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Beganović, Jasna ; Kos, Blaženka ; Gjuračić, Krešimir ; Šušković, Jagoda ; Frece, Jadranka
engleski
Genotypic and phenotypic diversity of lactic acid bacteria isolated from controlled sauerkraut fermentation
The increasing application of probiotic cultures in food products underscores the need to properly identify and distinguish these beneficial bacteria among originally presented microbial population. Probiotic strain Lactobacillus plantarum L4 and Leuconostoc mesenteroides LMG 7954 were introduced as starter cultures in sauerkraut production for improvement of fermentation process and product quality. Lower biogenic amine content and shorter duration of fermentation were obtained in production of sauerkraut with starter cultures. To check out the influence of starter culture strains, ten randomly chosen lactic acid bacteria isolates from controlled fermentation were genotyped by pulsed-field gel electrophoresis (PFGE), together with six reference strains for comparison. Electrophoretic karyotype of NotI-digested genomic DNA revealed 6 isolates as Lactobacillus plantarum, one was identified as Leuconostoc mesenteroides and one as possible Lactobacillus rhamnosus. The two remained isolates showed specific karyotype that could not be identified after comparison with reference strains. Results obtained by PFGE were confirmed by SDS-PAGE of whole cell proteins. Further phenotypic characterization of all strains examined was evaluated by studying biochemical characteristics (API carbohydrate fermentation patterns and acidifying activities) of technological interest. The results indicated that electrophoretic karyotype determined by PFGE allowed to confirm the identity of starter strains between other isolates and can be employed to study the genetic diversity of lactic acid bacteria from an ecosystem like that found in fermenting vegetables.
Lactobacillus plantarum; Leuconostoc mesenteroides; starter cultures; sauerkraut production; PFGE; SDS-PAGE; probiotics
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Podaci o prilogu
62-x.
2005.
objavljeno
Podaci o matičnoj publikaciji
Final Programme and Book of Abstracts of the Second Congress of Croatian Geneticists
Franekić-Čolić, Jasna ; Ugarković, Đurđica
Zagreb:
Podaci o skupu
Second Congress of Croatian Geneticists with international participation.
poster
24.09.2005-27.09.2005
Supetar, Hrvatska