Interaction of pyridinium oximes K027, K033 and K048 with native and tabun-inhibited human acetylcholinesterase (CROSBI ID 519471)
Prilog sa skupa u zborniku | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Kovarik, Zrinka ; Čalić, Maja ; Kuča Kamil ; Jun, Daniel
engleski
Interaction of pyridinium oximes K027, K033 and K048 with native and tabun-inhibited human acetylcholinesterase
This study examined the ability of three bispyridinium oximes K027 [1-(4-hydroxyiminomethylpyridinium)-3-(4-carbamoylpyridinium) propane dibromide], K048 [1-(4-hydroxyiminomethylpyridinium)-4-(4-carbamoylpyridinium) butane dibromide], and K033 [1, 4-bis(2-hydroxyiminomethylpyridinium) butane dibromide] to reactivate tabun-inhibited human erythrocyte AChE. Reversible inhibition of native AChE by these oximes and their protective index of AChE inactivation by tabun were determined as well. Tabun-inhibited AChE was completely reactivated by micromolar concentrations of K027 and K048. The reactivation by K033 reached 50 % after 24 hours. All the oximes were reversible inhibitors of AChE, and dissociation constants were 17 μ M, 73 μ M and 110 μ M for K033, K027 and K048, respectively. All studied oximes protected the enzyme from phosphonylation by tabun by at least factor 2. Our in vitro experiments pointed out K048 and K027 as promising reactivators, while K033 showed promise as a protective agent in tabun poisoning.
Acetylcholinesterase; Antidotes; Inhibition; Protection; Reactivation; Warfare nerve agents
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
173-175-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
The Proceedings of the Chemical and Biological Medical Treatment Symposium CB MTS - Industry IV "The Third World Congress on Chemical, Biological and Radiological Terrorism"
Bokan, Slavko ; Orehovec, Zvonko ; Price, Barbara
Aberdeen (MD): Applied Science and Analysis
Podaci o skupu
Nepoznat skup
predavanje
29.02.1904-29.02.2096