engleski

Aujeszky's disease virus production in disposable bioreactor

Novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky’ s disease (AD) virus production. Growth kinetics of BHK 21 C13 cells in the Wave bioreactor during 3 days to cell density of 1.82x106 cells ml-1 was determined. At the end of the 3 day culture period and cell density of 1.82x106 cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain AD virus suspension (105.9 TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of AD virus harvest was obtained with titre of 107.0 TCID50 ml-1, which corresponds to 40, 000 doses of vaccine against AD. In conclusion, the results obtained with the Wave bioreactor using BHK 21 C13 cells showed that this system can be considered as a suitable for AD virus or BHK 21 C13 cell biomass production.

Aujeszky’ s disease virus; baby hamster kidney cells; cell culture; disposable bioreactor; virus titre

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