engleski

Synbiotic effect of Lactobacillus acidophilus M92 and prebiotic substrates on the intestinal microflora of mice and their immune system

The ability of probiotic strain L. acidophilus M92 to assimilate the various kinds of prebiotic substrates (inulin, lactulose and raffinose) in vitro and in vivo, on Swiss albino mice as animal model, was investigated. Survival, competition, adhesion and colonization of L. acidophilus M92 in combination with prebiotics (synbiotic effect) were monitored in the gastrointestinal tract of mice, as well as their immunomodulating capability. After the feeding of mice with synbiotics (2 x 1010 probiotic cells in combination with 10% of prebiotic substrates) the number of lactic acid bacteria in faeces was increased and reduction of enterobacteria and sulphite-reducing clostridia was observed. The similar results were obtained in homogenates of small and large intestine of mice in the 1st and 14th day after feeding with synbiotics. After oral immunization of mice with viable cells of L. acidophilus M92, with a daily dose of 2 x 1010 cells, alone and in combination with prebiotic substrates, the concentration of faecal SIgA and total serum IgA antibodies from all immunized mice were significantly higher in comparison to the control. They didn’ t evoke the specific humoral immune response after oral application and they are as such suitable for synbiotic application. L. acidophilus M92 in combination with inulin has shown the best synbiotic effect on intestinal and faecal microflora of Swiss albino mice, and their immune system.

adhesion; Lactobacillus acidophilus; intestinal microflora; probiotic; prebiotic; synbiotic; inulin; lactulose; raffinose

nije evidentirano