Acetylcholinesterase and butyrylcholinesterase activity measurements in human blood by the Ellman method: II. Procedure protocol and comparison with procedures suggested by other authors (CROSBI ID 473006)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Reiner, Elsa ; Škrinjarić-Špoljar, Mira ; Šinko, Goran ; Simeon-Rudolf, Vera
engleski
Acetylcholinesterase and butyrylcholinesterase activity measurements in human blood by the Ellman method: II. Procedure protocol and comparison with procedures suggested by other authors
The procedure for plasma butyrylcholinesterase (BChE ; EC 3.1.1.8) activity measurement is well established, while this is not the case for the erythrocyte acetylcholinesterase (AChE ; EC 3.1.1.7). We suggest the separation of plasma from erythrocytes by centrifugation, followed by separate measurement (with 1.0 mM acetylthiocholine as substrate) of BChE in plasma and AChE in unwashed erythrocytes. The obtained results showed that the cholinesterase activity measured in whole blood equalled the summe of the AChE activity in suspended erythrocytes plus the BChE activity in plasma, calculated upon the measured haematocrit. In blood samples from 13 individuals, the summe of activities was 97 ą 7 % of the activity measured in whole blood. The above procedure was compared with the method of Portmann (CBMTS, Dubrovnik 1998) based on activity measurements with acetylthiocholine and butyrylthiocholine which distinguish AChE from BChE, and with the method of Szinicz et al. (CBMTS, Dubrovnik 1998) where AChE activities are measured with acetylthiocholine in presence of ethopropazine, a BChE inhibitor. The advantage of our procedure is that both enzymes are measured with the same substrate and no inhibitor is required for the AChE and BChE assays. Further, the procedure can be performed with commercial kits for plasma activity measurements.
acetylcholinesterase; butyrycholinesterase; activity measurement; human blood
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Podaci o prilogu
19-x.
2000.
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objavljeno
Podaci o matičnoj publikaciji
Technical Program, CB Medical Treatment Symposium: An Exploration of Present Capabilities and Future Requirements for Chemical and Biological Medical Treatment, Spiez, Abstracts of Platform and Poster Presenations
Price, Barbara
Spiez: AC-Laboratorium Spiez, Švicarska
Podaci o skupu
The Third Chemical and Biological Medical Treatment Symposium, Spiez, Švicarska
poster
07.05.2000-15.05.2000
Spiez, Švicarska