engleski

Probiotic strain Lactobacillus helveticus M92 reduces Salmonella enterica serovar Typhimurium infection in mice

The protective effects of the oral treatment with Lactobacillus helveticus M92 in an intragastric challenge by Salmonella enterica serovar Typhimurium were investigated. Mice were fed with milk-based diets supplemented with L. helveticus M92, for seven days prior to and following oral challenge with S. Typhimurium. Survival, competition, adhesion and colonization of L. helveticus M92 in combination with S. Typhimurium were monitored in the gastrointestinal tract of mice, as well as their immunomodulating capability. After feeding of mice with L. helveticus M92 the total number of lactic acid bacteria in faeces was increased, contrary the reduction of total number of enterobacteria was observed, compared to non-probiotic-fed mice (mice fed with S. Typhimurium). These results were in accordance with results obtained in homogenates of small and large mice intestine 7th day after feeding with L. helveticus M92. Following the oral immunization of mice, with a daily dose of 1 x 1011 viable L. helveticus M92 cells, the concentration of serum anti-S. Typhimurium IgA antibodies from all immunized mice was significantly higher compared to non-probiotic-fed mice i.e. mice fed with S. Typhimurium and to the control group of mice. Interestingly, results showed that, after the challenge, L. helveticus M92 – fed mice exhibited lower cumulative morbidity and bacterial translocation rates, compared to non-probiotic-fed mice. These results demonstrate that L. helveticus M92 can reduce the severity of infection due to the pathogen S. Typhimurium, and suggest that the reduction may be associated with enhanced immune protection conferred by the probiotic.

probiotic; Lactobacillus helveticus; Salmonella enterica serovar Typhimurium; infection; mice

nije evidentirano