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Alkali-soluble cell wall proteins of Saccharomyces cerevisiae (CROSBI ID 550796)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Stuparević, Igor ; Kovacs, Monica ; Maraz, Anna ; Mrša, Vladimir Alkali-soluble cell wall proteins of Saccharomyces cerevisiae // Power of microbes in industry and environment / Kosalec, Ivan ; Pigac, Jasenka ; Vujaklija, Dušica (ur.). Zagreb: Pressum, 2007. str. 138-138

Podaci o odgovornosti

Stuparević, Igor ; Kovacs, Monica ; Maraz, Anna ; Mrša, Vladimir

engleski

Alkali-soluble cell wall proteins of Saccharomyces cerevisiae

Yeasts have evolved three different ways regarding the glucan attached proteins of cell wall. Some proteins are bound noncovalently to the β -1, 3-glucan, while others are attached covalently to the β -1, 6-glucan through a GPI-anchor, or directly by alkali labile ester linkage between the γ -carboxyl group of glutamic acid and the hydroxyl group of glucose (named as Pir – proteins). Unusual versatility of the cell wall proteins implies different roles and reactions taking place in the wall. Different mutants lacking individual wall proteins or groups of proteins were constructed and their phenotypes were studied. After disruption of all the PIR genes (CCW5, CCW6, CCW7 and CCW8), a 67kDa protein still remained in NaOH extract. SCW4 disruption in the ccw5ccw6ccw7ccw8 mutant, however, resulted in disappearance of the 67kDa band from the extract, indicating that Scw4p could also be covalently linked to the cell wall, similarly to the Pir-proteins. Since it was reported previously that Scw4p was a noncovalently attached protein, this finding can be relevant for the role of Scw4p. A mild treatment of yeast cells with β -glucanase induced a drastic decrease in the cell surface hydrophobicity indicating that it is at least to a great extent due to mannoproteins of the outer layer of the cell wall. This phenomenon together with the obvious assumption that the outermost located mannoproteins play crucial role in the cell-cell interaction during film or floc formation motivated us to screen for the differences in cell wall protein composition between the wine yeast strains, which form a film (flor) on the wine surface and those unable to do so in order to get a better insight into the intermolecular interactions during cell attachment.

yeast; cell wall; Pir proteins; SCW4; biofilm

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Podaci o prilogu

138-138.

2007.

objavljeno

Podaci o matičnoj publikaciji

Power of microbes in industry and environment

Kosalec, Ivan ; Pigac, Jasenka ; Vujaklija, Dušica

Zagreb: Pressum

978-953-96567-5-9

Podaci o skupu

Power of Microbes in Industry and Environment

poster

19.09.2007-22.09.2007

Zadar, Hrvatska

Povezanost rada

Biotehnologija