engleski

Serum antibodies positivity to 12 Helicobacter pylori virulence antigens in patients with benign or malignant gastroduodenal diseases : cross- sectional study

To investigate the association of gastric histological and endoscopic findings in patients with H. pylori, according to presence of seropositivity to 12 bacterial virulence antigens. The study is a cross – sectional single center analysis of 360 consecutive outpatients referred in a single year to upper gastrointestinal endoscopy because of dyspeptic complaints. Patients sera were tested by Western blot method to determine the presence of serum antibodies to bacterial virulence antigens (p120 (CagA), p95 (VacA), p67 (FSH), p66 (UreB), p57 (HSP homolog), p54 (flagellin), p33, p30 (OMP), p29 (UreA), p26, p19, and p17). Upper gastrointestinal endoscopy was performed, endoscopic diagnosis recorded, and 4 mucosal biopsy samples obtained and assessed according to Updated Sydney protocol. The sera of 207 patients were analyzed. 30 patients had gastric adenocarcinoma, 126 peptic ulcers and 51 normal finding. P120 (CagA) seropositivity was significantly more often present in patients with higher activity grade in the antrum (P=0.025), p30 (OMP) with greater inflammation in the antrum (P=0.025) and the corpus (P=0.010), p33 with greater inflammation in the corpus (P=0.050) and p19 (OMP) with lower intestinal metaplasia grades in the corpus (P=0.025) of the stomach. Seroreactivity to all other bacterial proteins showed no association with the histological status of the stomach mucosa. Except for the seropositivity to protein p95 (VacA), which was more often present in patients with duodenal ulcer (P=0.006), there was no difference in seroreactivity to other bacterial proteins and upper gastrointestinal endoscopic findings. P120 (CagA), p33, p 30 (OMP) and p19 (OMP) seropositivity were more often present in patients with higher grades of the histological parameters of gastritis and seropositivity to protein p95 (VacA) with endoscopic presence of duodenal ulcer. Histological parameters of gastritis are rather associated with bacterial virulence than endoscopic findings.

H. pylori; gastritis; virulence antigens; CagA; VacA; p 19; p 30; p 33

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