engleski

Identification and quantification of phenolic compounds of sour cherries cv. Marasca (Prunus Cerasus Marasca) and their changes during freezing storage

Sour cherry marasca is important Croatian cultivar with limited cultivation only in the field of north and central part of Dalmatia. It is rich source of biologically active ingredients, particularly with phenolic compounds and anthocyanins. Sour cherries ecotype Sokoluša cv. Marasca from Zadar and Split growing area were analyzed at ripe stage and after freezing storage at -18°C for 6 months. Identification and quantification of phenolic compounds was performed by HPLC with UV/VIS PDA detector. Anthocyanins were dominated in all samples, and Sokoluša from Zadar growing area were more represented. The results showed that both sour cherries contained high amount of total anthocyanins (6.44 and 4.02 g/kg of dry matter). Cyanidin-3-glucosylrutinoside and pelargonidin-3-glucoside were major anthocyanins. Flavonols, hidroxycinnamates, flavan-3-ols and procyanidins were also determined but in significantly lower quantities and total values of previously mentioned compounds were higher in sour cherries Sokoluša from Zadar than from Split growing area. Besides anthocyanins flavonol glycosides were major phenolic compounds in analyzed samples (358.67 and 308.48 mg/kg of dry matter). Growing area has statistical significant affect on cyanidin-3-glucosylrutinoside, ferulic acid, kaemferol-3-glucoside, epicatehin, procyanidin 1 and on total flavanols and procyanidins. Cluster analysis showed that ecotype Sokoluša from different growing area is possible distinguished in accordance with determined compounds. Generally, freezing storage caused decreasing of anthocyanins and phenolics quantities except total flavonols in sour cherries from Zadar area and has statistical significant affect on ferulic acid, procyanidin 1 and on total flavanols and procyanidins.

anthocyanins; freezing; phenolic compounds; sour cherry Marasca

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