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Atrazine toxicity on the rat prostate subcellular mechanisms (CROSBI ID 464013)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Jakominić, Mihela ; Šimić, Branimir ; Kniewald, Zlatko ; Kniewald, Jasna Atrazine toxicity on the rat prostate subcellular mechanisms // XXXVI European Cogress of Toxicology - EUROTOX"97 Pharmacology & Toxicology, Volume 80, Supplement III, 1997 / The Danish Society of Pharmacology and Toxicology (ur.). Aarhus: Munksgaard, 1997. str. 109-110-x

Podaci o odgovornosti

Jakominić, Mihela ; Šimić, Branimir ; Kniewald, Zlatko ; Kniewald, Jasna

engleski

Atrazine toxicity on the rat prostate subcellular mechanisms

Interaction between ^3H-5alpha-dihydrotestosterone and its specific nuclear receptors (NR) in rat prostate (P) under atrazine (A) influences were studied. Scatchard plots for specific binding revealed K_d value 2.5 x 10^-12 M and binding sites" of 422 fmol/mg of DNA. In vitro 0.7 micromol of A induces decrease in number of binding sites on NR molecules (286 fmol/mg of DNA), while K_d remains in the same range. Protein contents in P cytosol were followed in vivo treatment per os with 8 or 12 mg A per kg b. w./10 days. Proteins in P cytosol were resolved by 1-D 12% SDS PAGE after Commassie blue staining and quantified by UltroScan XL densitometer. The decreases from 6 to 44% in protein contents at Mr 10.5 kD, 12.8 kD, 15.1 kD, 21.7 kD, 31.6 kD, 50.9 kD and 62.3 kD were detected vs. controls. The highest changes in protein band contents were detected at Mr 31.6 kD and 50.9 kD in samples from the treated rats with the higher dose. The study present findings in subcellular changes under A influence at rat P level, which is one of the most widely used experimental systems for studying the androgen gonadotropic feedback regulation.

atrazine; male rat; prostate; nuclear receptors

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Podaci o prilogu

109-110-x.

1997.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

XXXVI European Cogress of Toxicology - EUROTOX"97 Pharmacology & Toxicology, Volume 80, Supplement III, 1997

The Danish Society of Pharmacology and Toxicology

Aarhus: Munksgaard

Podaci o skupu

XXXVI European Cogress of Toxicology - EUROTOX"97

poster

25.06.1997-28.06.1997

Århus, Danska

Povezanost rada

Prehrambena tehnologija