engleski

REMAP as a Tool for Preliminary Grapevine Accession Screening

One of the main problems in management of germplasm collections of perennial crops such as grapevine is the occurrence of synonyms and homonyms among accessions. Traditional identification procedures based on morphological characters are not consistent and reliable enough. Genetic identification using DNA-based markers have been used for more reliable cultivar identification and microsatellite markers (SSR) have been commonly used for grapevine genotyping. Although SSRs are a codominant, highly reproducible and informative marker system they are rather laborious. Traditional electrophoresis systems (polyacrylamide gels followed by silver staining) are being used or in the case of capillary electrophoresis, fluorescently labeled primers cause higher price of analysis per sample. In addition, as previously published at least six SSR loci should be analyzed for reliable grapevine identification. A set of Vitis vinifera L. cultivars were chosen to test the discriminating power of REtrotransposon-Microsatellite Amplified Polymorphism (REMAP) using Gret1 LTR primers in comparison to a common set of SSR loci. All of the tested cultivars could be distinguished by using only one or two primer combinations on agarose based electrophoresis resulting in a cheap and useful tool for preliminary accession screening.

germplasm; accession screening; SSR; REMAP

Rad je kao predavanje prezentiran na skupu International Symposium on Molecular Markers in Horticulture, održanom od 29.07-01.08.2010., Corvallis, Oregon, USA ; ISBN 978-90-66052-28-4.