engleski

Study of different growth promotors on BHK 21 C13 biomass production

Summary: BHK21 C13 cells were used as a model in production of vaccine against Aujeszky diseases. In order to obtain sufficient cell concentration for different sera and growth promotor CABRPP were examined. CABRPP gave the best result and its application can significantly reduce the ammount of necessary serum. Materials and Methods: Baby hamster kidney cells (BHK21 C13, ATCC CCL 10 stored at liquid N2 were used for biomass production. Cell propagation has been performed after thawing at 37 degree C with medium + 10 percent serum. Further biomass production has been performed at T-flasks and cell spin bioreactor under controlled atmosphere. Influence of two different media (MEM N and Glasgow BHK21 Medium), four different sera (Horse serum, Fetal calf serum, Donor calf serum, and Newborn calf serum). Three different initial cell concentrations and influence of growth promotor CABRPP were examined. Overview and discussion of results: During cell growth the total cell density, viability and growth kinetics were determined. The best results were obtained with Glasgow BHK21 medium, supplemented with 5 percent horse serum and initial cell concentration of 3.6 x 10exp.5. Total cell density was 1.6 x 10 exp.6, viability over 95 percent and tD= 16 h. In the case when the part of serum was replaced with the growth promotor CABRPP, while the total protein content remained the same, total cell density increased for 50 percent (2,4 x 10exp 6),viability remained over 95 percent and tD reduced for 33 percent (tD 12 h.). Vaccine production with such cells has shown the same or better activity when compared with traditional procedure. Conclusions: Addition of CABRPP increases total cell density (50 percent) and reduces )tD 33 percents). Also CABRPP reduces amount of necessary serum addition.

animal cells; high cell density; growth promoters; BHK 21 C13 cells

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