engleski

Aflatoxin Accumulation During the Growth of Mould Aspergillus flavus ATCC 26949 on Corn in Pure and Mixed Culture as Related to Various Temperature and Moisture Content.

The relationship between mould biomass and the biosynthesis of aflatoxin B1 and G1 on solid substrates(whole and crushed maize grain) at temperatures from 15-40 oC and a water content in the substrate of 20-38% has been investigated. The experiments have been carried out with the mould Aspergillus flavus ATCC 26949 in pure culture and in mixed culture respectively, the latter with the mould Trichothecium roseum ZMPBF 1226. The biomass growth during cultivation was measured by the chitin content, and the concentration of aflatoxins was determined by means of a ELISA. It has been established that the biosynthesis of the examined aflatoxins and their ratio primarily depend on the temperature of cultivation, rather than on the growth of the mycelium. The biomass of the mixed culture of A. flavus and T. roseum after 35 days of cultivation reduces the amount of aflatoxin B1 by 19-40%, and the amount of aflatoxin G1 by 30-45%. The decrease of concentration of both toxins is more pronounced in the substrate with a higher initial water content and at a higher temperature of cultivation.

Moulds; mixed cultures; mycotoxins; ELISA

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