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Salmonella Typhimurium systemic disease in mice

Salmonella enterica causes a wide variety of disease syndromes in humans, many of which are associated with significant levels of fever. Typhoid fever is an invasive enteric infection in which viable bacteria can often be isolated from the blood of infected individuals. Since S. Typhi does not cause significant disease in animals, murine typhoid fever, caused by certain S. enterica serotypes including Salmonella Typhimurium, has been used extensively as a model for systemic salmonellosis. The purpose of this study was to determine the exact time when the bacteria disseminate from small intestine to liver. Pathogen free, male, 8 to 12 weeks old Balb/c mice were infected per os with Salmonella Typhimurium, using dose of 105 cfu. The number of bacteria in small intestine and liver was determined at different time points. The histopathological changes in small intestine and liver were also followed. Increasing multiplication of S. Typhimurium in small intestine was associated with increast colony counts in small intestine. In the liver, bacteria were detected on 3th day post infection. Histopathology of liver and small intestine showed inflammatory cellular infiltrates. We confirmed that S. Typhimurium rapidly disseminate in the liver causing systemic disease in mice.

Salmonella; mice model; typhoid fever; systemic disease

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