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Using S. cerevisiae cell wall protein Ccw12p as a anchor for expression of two yeast nucleases at the cell surface (CROSBI ID 570440)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Teparić, Renata ; Mrša, Vladimir Using S. cerevisiae cell wall protein Ccw12p as a anchor for expression of two yeast nucleases at the cell surface // The secret life of biomolecules HBDMB2010 / Kovarik, Zrinka ; Varljen, Jadranka (ur.). Rijeka: Hrvatsko Društvo za Biotehnologiju, 2010

Podaci o odgovornosti

Teparić, Renata ; Mrša, Vladimir

engleski

Using S. cerevisiae cell wall protein Ccw12p as a anchor for expression of two yeast nucleases at the cell surface

In the past several years a number of surface – engineered yeasts, displaying different heterologous proteins interesting for biotechnological or medical applications, have been constructed. Yeast cell surface systems for the display of heterologous proteins have the advantages of simplicity of genetic manipulation, ability for proper post-translational modifications and folding of mammalian proteins and immobilization of heterologous proteins covalently under mild, physiological conditions without risk of damage by chemical treatment. S. cerevisiae cell wall proteins that are covalently bound to the carbohydrate components of the wall can be divided in two main groups. Majority of proteins of this class are bound at their C-termini through a remnant of the GPI-anchor. A smaller group of proteins are directly covalently bound at their N-termini to -1, 3-glucan by the alkali labile ester linkage between the glutamic acid γ-carboxyl group and hydroxyl groups of glucoses (Pir–proteins). In this work Ccw12p cell wall protein, that belongs to the group of cell wall proteins bound through GPI-anchor remnant, is used for C-terminal immobilization of two yeast intracellular nucleases, Yblp and Rnyp, to the yeast cell surface.

yeast cell wall; Ccw12p; heterologous expression

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Podaci o prilogu

2010.

objavljeno

Podaci o matičnoj publikaciji

The secret life of biomolecules HBDMB2010

Kovarik, Zrinka ; Varljen, Jadranka

Rijeka: Hrvatsko Društvo za Biotehnologiju

Podaci o skupu

10th congress of the Croatian society of biochemistry and molecular biology

poster

15.09.2010-18.09.2010

Opatija, Hrvatska

Povezanost rada

Biotehnologija