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DETERMINATION OF TOTAL PHENOLIC COMPOUNDS IN CULTIVARS OF CASTANEA SATIVA MILL.

The cultivar of the sweet chestnut, Lovran's ‘Marrone’ and grafted Italian ‘Marrone’ have been investigated in this study. The analysis were done with different parts of sweet chestnut, such as: peeled seed, brown shell, red seed coat, seed, leaf, catkin, spiny bur, as well as the new and old chestnut bark, which were extracted by 50% ethanol, as well as by 50% acetone, as an extragents. The plant material was milled to an average particle size of 0.18 to 0.40 mm. The yield of dry extract, expressed as % (w/w), measured from 1.82% for spiny burs (extragent 50% ethanol) to 33.56% for catkins (extragent 50% acetone). The total phenolic compounds content obtained in dry extracts was determined using the Folin – Ciocalteu assay and expressed as g of gallic acid equivalents (GAE) per 100 g of the dry extract sample (% ; w/w), i.e. % gallic acid equivalents (GAE). That value was from 0.42 %GAE for the seed (extragent 50% ethanol) to 6.89 %GAE for the leaf (extragent 50% acetone). The lowest content of %GAE (0.11) were found on peeled seed (extragent 50% ethanol), and the highest (11.42 %GAE) on catkins (extragent 50% acetone). The %GAE value of grafted Italian "Marrone" cultivar was the lowest (0.41 %GAE) for the sample of the whole fruit (extragent 50% ethanol) and the highest (6.45 %GAE) has obtained for the leaf (extragent 50% acetone). The studied sweet chestnut cultivars are important natural antioxidants for use as food ingredients.

Cultivar; dry extract; extragent; natural antioxidants; sweet chestnut

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