Interaction of silver nanoparticles with cholinesterases (CROSBI ID 574567)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Šinko, Goran ; Vinković Vrček, Ivana ; Miljanić, Snežana ; Dijanošić, Adriana
engleski
Interaction of silver nanoparticles with cholinesterases
The environmental impact of nanomaterials is expected to increase substantially in the near future as they are becoming a part of our daily life. Silver nanoparticles (AgNP) are widely used in the form of cosmetics, food packaging, drug delivery systems, therapeutics, antimicrobial agents, biosensors, or labels. Manufactured nanoparticles can be toxic via interactions with proteins and enzymes. Nanoparticles are known to be able to interfere with cellular metabolism and to cause cytotoxicity and moreover may interfere with specific cellular functions. We studied interaction of AgNP with acetylcholinesterase (AChE) and related butyrylcholinesterase (BChE). Both cholinesterases are present in brain, blood and nervous system. Acetylcholinesterase (AChE) is a key enzyme in cholinergic neurotransmission and inhibition of AChE may lead to fatal outcome. BChE has important pharmacological and toxicological roles due to its hydrolysis of various xenobiotics. We have evaluated the ChE inhibition by AgNPs prepared using reduction methods of silver nitrate with trisodium citrate (AgNP-citrate), hydroxylamine hydrochloride (AgNP-chloride) and sodium borohydride in presence of polyvinyl alcohol (AgNP-PVA). All tested AgNPs inhibited ChEs and the evaluated apparent inhibitor constants ranged from 49 μM to 670 μM. In the case of AChE the inhibition potency increased in the following order: AgNP-PVA < AgNP-citrate < AgNP-chloride, while in the case of BChE the inhibition potency increased in the following order: AgNP-citrate < AgNP-PVA < AgNP-chloride. The impact of AgNP on ChE activity depends greatly on the nature of the nanomaterial coatings. It is also interesting that AgNP showed higher affinity for AChE, than for BChE.
silver nanoparticles; acetylcholinesterase; butyrylcholinesterase; inhibition
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Podaci o prilogu
S433-x.
2011.
objavljeno
Podaci o matičnoj publikaciji
Abstracts of the 36th FEBS Congress, Biochemistry for Tomorrow's Medicine ; in: The FEBS Journal 278 (2011) (S1)
Wiley-Blackwell
1742-464x
Podaci o skupu
36th FEBS Congress, Biochemistry for Tomorrow's Medicine
poster
25.06.2011-30.06.2011
Torino, Italija