Separation and identification of cuticular fractions of dried tomato by-products (CROSBI ID 581255)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Ninčević Grassino, Antonela ; Grabarić, Zorana
engleski
Separation and identification of cuticular fractions of dried tomato by-products
Tomato (Lycopersicon esculentum Mill) is one of the most widely cultivated vegetable crops in Mediterranean countries. Significant amounts are consumed in the form of processed products, i.e. tomato juice, paste, purée, ketchup, sauce and salsa. The commercial processing of tomato results in the accumulation of large amounts of waste, composed by mixture of tomato skin, pulp and seeds. The cuticle of these by-products is chemically heterogeneous in nature consisting mainly of a wax fraction, soluble in organic solvents, and an insoluble lipophilic matrix, namely cutin. This high-molecular weight polyester has a very low water sorption and permeability, high specific heat and as such could be a potential substrate for biofilm production. The present work reports methodology of cuticular fractions (bulk, dewaxed and nonhydrolyzable cuticles) extraction and purification from dried tomato skin. For that purpose, the tomato skin is firstly treated with acid/ammonium oxalate solution at 90 °C for 24 h to remove the pertinacious glue that attaches the cuticle to the epidermal layer. After that dewaxed fraction is obtained after exhaustive Soxlet extraction with various organic solvents (chloroform, dichloromethane, petroleum ether and methanol), to obtain the best extraction yield. Subsequently, the dewaxed tomato skin was hydrolysed with 6 M HCl under reflux, to obtain the pure cutin biopolymer. The obtained fractions were characterised using FTIR spectroscopy. The FTIR spectra of dewaxed cuticular fractions indicate the presence of two strong absorption bands located at 2916 and 2848 cm-1, associated with the asymmetric and symmetric stretching vibrations of the methylene groups and relatively strong bands at 1745 and 1162 cm-1, assigned to the carbonyl stretching of the carboxyl group and C-O-C stretching vibrations in the ester group. The hydrolysis of dewaxed cuticular fraction with 6 M HCl caused the ester bonds in cutin polymer to break, corresponding to removal of hydroxy fatty acids. The results of FTIR spectroscopy confirm that all cuticular fractions were successfully isolated from dried tomato skin and that this spectroscopic technique can be used as excellent tool for characterisation and validation of tomato by-products. Further investigations should be focused on modification of extractions procedure to avoid large volumes of organic solvents, which may cause environmental problems.
cutin; cuticular fractions; extraction; FTIR spectroscopy; tomato byproducts
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Podaci o prilogu
152-152.
2011.
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objavljeno
Podaci o matičnoj publikaciji
Current opinion in biotechnology
Dundar, Munis ; Gahan Peter B ; Magni, Maria Pia
Elsevier
0958-1669
Podaci o skupu
European Biotechnology Congress 2011
poster
28.09.2011-01.10.2011
Istanbul, Turska