engleski

Production of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), Biomass and Polysaccharides by Solid State Cultivation

Solid state cultivation of Ganoderma lucidum biomass, strain MZKI G97 originally isolated from the Slovenian forest, was studied in horizontal stirred tank reactor. Periodically mixing of N = 80 rpm, 2 minutes/day was used. Production of fungal polysaccharides and fungal biomass on solid substrate based on beech saw-dust, olive oil and mineral salts, was studied. Optimal moisture of the solid matrix in the range of 80 to 74 % was found. When the moisture content dropped below 57 %, the growth of the mycelium and polysaccharide production stopped, but it revived when wet air was applied in further process. Final concentration of biomass was 0.68 mg/g of solid substrate, while portions of extracellular and intracellular polysaccharides were 4.5 mg/g and 1.05, respectively.

medicinal mushrooms; Ganoderma lucidum; solid state cultivation; horizontal stirred tank reactor; fungal biomass; critical moisture content; polysaccharide production

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