Determination of glucose in croatian wines by amperometric biosensor and spectrophotometrically (CROSBI ID 596443)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Ninčević Grassino, Antonela ; Beneti, Lucija ; Fakin, Žana ; Grabarić, Zorana ; Grabarić Božidar S.
engleski
Determination of glucose in croatian wines by amperometric biosensor and spectrophotometrically
So far we developed amperometric biosensors for glucose determination based on glucose oxidase (GOx) enzyme in many different ways and applied them for various analytes in food anlysis [1, 2]. In this work GOx was immobilized on the surface of modified graphite electrode by entrapment into palladium hexacyanoferrate (PdHCF) hydrogel and the biosensor prepared in this way was used for determination of glucose in Croatian wines using standard addition method. The electrochemistry of enzyme-modified electrode was investigated by cyclic voltammetry and amperometry under batch conditions. The response of biosensor was tested as a function of selected organic solvents (methanol, ethanol, acetone and acetonitrile) containing phosphate buffer solution (pH = 5.5) at -50 mV against a reference HgHg2Cl23 M KCl electrode. The same samples were, for comparison, analysed with the spectrophotometric method as well, based on two stage enzymatic reactions which results, after oxidation of glucose, in formation of a coloured potassium hexacyanoferrate(III) in solution. The results have shown that NiHCF as redox mediator offer better electrocatalytic efficiency for hydrogen peroxide reduction compared with bare graphite electrode. Addition of organic solvents (φ = 10–60 %) decreased the biosensor response, therefore the determination of glucose in Croatian wines has been performed in phosphate buffer (pH = 5.5). The linear range was obtained in the concentration range from 0.1 to 0.6 mM with a squared correlation coefficient of 0.9994. Correlation between the results obtained by the GOx biosensor and spectrophotometrically was acceptable although the biosensor determination is much more rapid. The proposed amperometric GOx biosensor has been successfully applied for determination of glucose in some red and white Croatian wines. Its analytical performance in terms of high activity, long life and reasonable accurate glucose determination can be recommended in food industry for quality control of wines as well as during fermentation process, when glucose is transformed into alcohol.
amperometric biosensor; glucose; Croatian wines
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Podaci o prilogu
39-39.
2012.
objavljeno
Podaci o matičnoj publikaciji
Jukić, Ante
Zagreb : Osijek: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku
Podaci o skupu
14. Ružičkini dani
poster
13.09.2012-15.09.2012
Vukovar, Hrvatska