Expression of sodium-D-glucose cotranspotre SGLT1 (SLC5A1) in murine organs (CROSBI ID 598603)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Vrhovac, Ivana ; Breljak, Davorka ; Koepsell, Hermann ; Sabolić, Ivan
engleski
Expression of sodium-D-glucose cotranspotre SGLT1 (SLC5A1) in murine organs
Previous studies have demonstrated a high affinity/low capacity sodium-D-glucose cotransporter Sglt1 (Slc5a1) in the brush-border membrane (BBM) of small intestine absorptive cells and proximal tubule (PT) epithelial cells, where Sglt1 mediates glucose (re)absorption. However, the detailed localization of mouse Sglt1 (mSglt1) along the nephron, and its cellular distribution in other organs is unknown. Here, we compared wild-type (Sglt1+/+) and Sglt1 knock-out (Sglt1-/-) mice in order to describe the cell localization of mSglt1 protein and mRNA expression in various organs. Localization of mSglt1 protein was analyzed by immunocytochemistry (IC) and Western blotting (WB) using the anti-mSglt1 antibody whose specificity was previously confirmed in Sglt1 knock-out mice. The expression level of mSglt1 mRNA was analyzed by RT-PCR. mSglt1 protein was localized in the BBM of PT, where it exhibited segmental (S2>S3) and zonal (cortex>outer stripe) differences, in the apical domains of thick ascending limb of Henle, liver bile ducts, and pancreatic ducts. This staining was not observed in the respective organs of Sglt1 knock-out mice. RT-PCR data were comparable to IC data. By WB, a single mSglt1-related protein band of 75 kDa was detected in the kidney BBM. By IC and RT-PCR, mSglt1 expression was not detected in the brain, spleen and skeletal muscle. This study provides new insight into mSglt1 expression in various mouse organs, thus enabling a better understanding of the role of mSglt1 in future physiological, pathophysiological, pharmacological, and toxicological studies.
bile duct; immunocytochemistry; intestine; liver; kidney; knock-out mice; membrane transporter; mRNA expression; pancreas; proximal tubules; RT-PCR; Western blotting
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Podaci o prilogu
351-351.
2013.
objavljeno
Podaci o matičnoj publikaciji
Arhiv za higijenu rada i toksikologiju 64(2)
Kopjar, Nevenka
Zagreb: Institut za medicinska istraživanja i medicinu rada
0004-125454
Podaci o skupu
1. hrvatski simpozij o transporterima SOT-1
predavanje
06.06.2013-07.06.2013
Zagreb, Hrvatska