engleski

EXPRESSION AND LOCALIZATION OF THE CHLORIDE/FORMATE EXCHANGER Slc26a6 (CFEX, PAT-1) IN RAT ORGANS

CFEX in various mammalian organs exchanges anions, such as chloride, bicarbonate, oxalate, formate, and hydroxyl ion. The tissue expression and localization of CFEX protein has been studied in mice, but its presence in rat organs is poorly documented. Here we used an anti-CFEX antibody (CFEX-Ab) to study the expression of this transporter in the kidneys, liver, gastrointestinal tract, and pancreas of adult Wistar rats of both sexes. In the kidneys, possible sex-dependent expression of this protein was studied in more detail. The experiments were performed by immunofluorescence cytochemistry (IC) in tissue cryosections, and by Western blotting (WB) of total cell membranes (TCM) isolated from the tissues. By IC, the CFEX-Ab stained with heterogeneous intensity the brush border membrane of renal proximal tubules (PT ; S1~S2>S3) and intestinal enterocytes (duodenumjejunum), the canalicular membrane of hepatocytes, and the apical cell membrane of pancreatic ducts. By WB of TCM, the antibody labeled a protein band of 100 kDa. In PT, the expression of CFEX protein was stronger in males, downregulated by castration, and unaffected by ovariectomy. In conclusion, the CFEX protein was detected in all tested rat organs. In the kidneys, the male-dominant expression of this transporter is caused by androgen stimulation.

CFEX; rat; western blotting; immunocytochemistry

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