engleski

Expression of aminpeptodase N (APN ; EC 3.4.11.2 ; CD13) and neutral endopeptidase (NEP ; EC 3.4.24.11 ; CD10) on cultured human keratinocytes

By secreting cytokines and growth factors keratinocyte actively participate in immune response and inflammation. Membrane-bound aminopeptidases have recently been proposed as additional mechanism of cell-to-cell interaction, as well as a negative loop in controlling concentration of peptide signalling molecules. The expression of two membrane-bound peptidases: aminopeptidase N (APN ; EC 3.4.11.2 ; CD13) and neutral endopeptidase (NEP ; EC 3.4.24.11 ; CD10) on nonstimualted cultured human keratinocytes obtained from normal skin was assessed. Membrane expression of CD13 and CD10 at the population level was analysed by FACS and at the single cell level by means of fluorescent microscope. Functional properties of CD13 and CD10 were examined by testing their enzymatic activity in the presence of selective substrates and inhibitors. The data were compared to those obtained on cultured nonstimulated human skin fibroblasts expressing both CD13/APN and CD10/NEP. Cultured, nonstimulated keratinocytes from healthy human skin expressed CD13, associated with APN enzyme activity. However, neither membrane CD10, nor its NEP activity could not be detected on the same keratinocyte samples. Presence of CD13 with catalytic activity on keratinocytes may be relevant for their communication with surrounding cells in the course of immune response and inflammation.

aminopeptidase N; neutral endopeptidase; cultuted keratinocytes

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