Adaptation of permanent fish cell line CCO for growth in serum-free medium (CROSBI ID 604047)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gaurina Srček, Višnja ; Radošević, Kristina ; Dukić, Bogdanka
engleski
Adaptation of permanent fish cell line CCO for growth in serum-free medium
The use of fish cell lines in biotechnology, ecotoxicology, virology and basic researches is constantly increasing. Proliferation of fish cells in culture is dependent on factors usually presented in serum of animal origin. Due to many obstacles regarding serum usage, serum-free media (SFM) are preferred, especially in the bioprocesses using animal cells. The aim of this study was to adapt fish CCO cells to grow in SFM by gradual reduction of serum content. During adaptation in UltraCulture medium (UCM) with 10%-0% (v/v) foetal bovine serum (FBS), obtained cell yields were in the range of 17.8-2.8x10e5 cells/ml. The cryopreserved SFM-CCO cells were checked during 3 months of storage and could be revived with cell monolayer established within one week. Since CCO cells are used for Channel Catfish Virus (CCV) vaccine production, we also attempted to obtain higher cell yields in low-serum media. CCO cells adapted to 2% of FBS were cultivated on Cytodex 1 microcarriers in Cell-spin bioreactors for 13 days. Results showed slightly higher cell yield (17.0x10e5 cells/ml) in UCM with 2% FBS when compared to regular DMEM medium with 5% FBS (15.0x10e5 cells/ml). In conclusion, CCO cells were successfully adapted to grow in SFM but further optimization is needed for their efficient use in microcarriers/bioreactor system.
adaptation; CCO cells; serum-free medium
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Podaci o prilogu
S63-S63.
2013.
objavljeno
Podaci o matičnoj publikaciji
European Biotechnology Congress 2013 - Current Opinion in Biotechnology
Van der Meer, Jan ; Stephanopoulos, Greg N.
Bratislava: Elsevier
0958-3669
Podaci o skupu
European Biotechnology Congress 2013
poster
16.05.2013-18.05.2013
Bratislava, Slovačka