engleski

Mass spectrometry based clinical proteomics

Rapid sequencing of peptides using MALDI-TOF is still a challenge since it is considered as a critical capability in modern proteomics research. It is a tool of utmost importance for rapid identification of pathogenic cells, as well as for high throughput characterization of cell-based products in pharmaceutical and food processing industry. Coupling this technology with newly developed computer software it is possible to deliver new services capable of "one step" identification of target proteins in a microorganism or a cell. In order to achieve this goal, we propose a faster, cheaper, more selective and reproducible analytical method for the determination of amino acid sequence of peptides (triptic digests) by derivatisation with disulphonic acid (Patent protected technology of Ruđer Bošković Institute) coupled with novel software developed at Faculty of Food Technology and Biotechnology. Above mentioned methods applied to proteomic analyses could eventually be applied in clinical practice as fast, reliable and efficient tools for microorganism detection and identification.

Proteomics research ; rapid sequencing of peptides ; MALDI-TOF ; rapid identification of pathogenic cells ; computer software

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