Proteolytic processing of Saccharomyces cerevisiae cell wall proteins Scw4p and Scw10p (CROSBI ID 611453)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Teparić, Renata ; Grbavac, Antonija ; Čanak, Iva ; Mrša Vladimir
engleski
Proteolytic processing of Saccharomyces cerevisiae cell wall proteins Scw4p and Scw10p
Yeast cell wall contains proteins that are noncovalently (Scw-proteins) or covalently (Ccw-proteins) bound to β-1, 3-glucan, the latter either through GPI-anchors and β-1, 6- glucan, or by alkali labile ester linkages between γ-carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir-proteins, extracted from the cell wall by mild alkali). It was previously shown that one of the most abundant Scw protein, Scw4p is partly also covalently linked to the cell wall, as well as it’s homolog Scw10p. In this work it was shown that part of Scw4p and Scw10p underwent the proteolytic processing resulting in three forms of the protein in the cell wall. The proteolytic enzymes which might have a role in processing of Scw4p and Scw10p are Kex2p and a family of aspartic proteases called yapsins. To get a better insight in the processing of these proteins, different mutations were introduced to SCW4 in the region of predicted processing sites. Native and mutated forms of Scw4p were expressed in kex2 yeast strain and strain with all yapsin genes disrupted (5yps∆) and Scw4p processing was examined. Protein glycosilation was also examined in order to determine whether forms differ in this way.
yeast cell walls; cell wall proteins; Scw4p; Scw10p; Kex2p
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
44-44.
2013.
objavljeno
Podaci o matičnoj publikaciji
Power of Microbes in Industry and Environment 2013
Teparić, Renata ; Frece, Jadranka ; Mrša, Vladimir
Zagreb: Recedo
978-953-7778-06-4
Podaci o skupu
Central European Symposium on Industrial Microbiology and Microbial Ecology "Power of Microbes in Industry and Environment 2013"
predavanje
09.10.2013-12.10.2013
Primošten, Hrvatska