engleski

The use of conventional and molecular methods for the routine confirmation of Listeria monocytogenes

The purpose of this research was to determine whether the unpasteurised milk, fresh cheese and cream of raw milk that are pro¬duced in the traditional manner at home and are free to sale on Zagreb markets, meet microbiologi¬cal requirements for foodstuffs. Particular attention is given to research of bacteria Listeria monocytogenes presence in these foods, because of its exceptional hazards to human health. Conventional (microbiological and biochemical – API test) and PCR methods were used to detect L. monocytogenes in these products. Of the 180 milk products samples tested, 27.6% were presumptively positive for Listeria on PALCAM agar. Only 21.3% of samples were confirmed to be positive for Listeria by API Listeria test, and 17.3% were confirmed to be positive for L. monocytogenes by PCR amplification of the hly gene (64 bp). PCR was able to eliminate the false positive and detect all L. monocytogenes in the milk products, unlike the conventional methods used in the industry. PCR proves to be a sensitive and rapid technique to be included in the procedure of detection of L. monocytogenes in food products and this method is considerably faster than current standard methods.

Listeria monocytogenes; PCR; milk products.

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