engleski

DEVELOPMENT OF HPLC METHOD FOR MONITORING ACID- CATALYZED CONVERSION OF 7-ETHYLTRYPTOPHOL TO METHYL ESTER OF ETODOLAC

Etodolac methyl ester is a key intermediate in the synthesis of Etodolac, a non-steroidal anti-inflammatory drug. It can be obtained by oxa-Pictet-Spengler reaction, starting from 7- ethyltriptophol and using inorganic mineral acids as catalysts.[1] In order to get kinetic profiles of reactions catalyzed with different molar ratios of sulfuric and hydrochloric acid, a high performance liquid chromatography method with UV detection was developed for monitoring conversion of 7-ethyltryptophol to methyl ester of Etodolac. For that purpose different stationary phases (C18, Phenyl and NH2 column) were investigated for separation of 7-ethyltryptophol and Etodolac methyl ester. Elution was performed with three different solvent mixtures in isocratic and/or gradient mode. The best chromatographic separation was achieved isocratically using Waters Symmetry Shield RP 18 column with 55% acetonitrile and 45% water.

HPLC method; kinetic study; Oxa Pictet Spengler reaction

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