Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry (CROSBI ID 221228)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Jurica, Karlo ; Brčić Karačonji, Irena ; Šegan, Sandra ; Milojković Opsenica, Dušanka ; Kremer, Dario Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry // Arhiv za higijenu rada i toksikologiju, 66 (2015), 3; 197-202. doi: 10.1515/aiht-2015-66-2696

Podaci o odgovornosti

Jurica, Karlo ; Brčić Karačonji, Irena ; Šegan, Sandra ; Milojković Opsenica, Dušanka ; Kremer, Dario

engleski

Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry

The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R2>0.9987) in the tested concentration range (0.5-200 μg mL-1), as well as good precision (RSD<5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 μg mL-1 for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Koločep (6.82 mg g-1 dry weight) was found to be higher (tpaired=43.57, tc=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Lošinj (2.75 mg g-1 dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).

Ericaceae ; food safety ; GC-MS ; HPLC ; solvent extraction

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

66 (3)

2015.

197-202

objavljeno

0004-1254

1848-6312

10.1515/aiht-2015-66-2696

Povezanost rada

Kemija, Biologija, Farmacija

Poveznice
Indeksiranost