engleski

Comparative study on cytogenotoxic effect of apigenin on tumour and non-tumour cells

Apigenin, one of the most common flavonoids found in a variety of fruits, leafy vegetables as well as tea and wine, has been shown to possess anti- oxidant, anti-inflammatory and anti-cancer properties. In this study, hormone responsive, ER-positive MCF-7 and triple-negative MDA MB-231 breast tumour cells were used to investigate potential cytogenotoxic effect of apigenin as well as the type of cell death in vitro. Based on our results, apigenin treatment induced changes in cell morphology in dose- (10-100 µM) and time- dependent manner. Moreover, apigenin caused cell death in both cell lines (with MCF-7 cells being more sensitive) leading to significant toxicity, which was evaluated by MTT and XTT assays. Additionally, apigenin induced DNA damage in both cell lines in dose-dependent manner with MDA MB- 231 cells being more sensitive compared to MCF-7 cells based on the comet assay results. Differential staining using acridine orange/ethidium bromide indicates apoptosis as a dominant type of cell death in both cells. This was also confirmed by Western blot analysis which detected cleaved PARP (poly(ADP-ribose) polymerase), as a marker of apoptotic cell death, after treatment with apigenin in concentrations of IC50 and above. Cytogenotoxic effect of apigenin was also evaluated on non-tumour circulating blood cells, human lymphocytes. According to our results, apigenin showed no effect on lymphocyte viability and there was no significant DNA damaging effect observed as determined by the comet assay, indicating its safety from the aspect of cytogenotoxicity towards normal human cells. Observed cytogenotoxic and pro-cell death activities of apigenin coupled with lack of toxicity (concentrations up to 100 µM) towards non-tumour cells, indicates that this natural product could be a useful candidate in the future breast cancer therapy.

MTT ; MCF-7 cells ; MDA MB-231 cells ; human lymphocytes ; comet assay

nije evidentirano