engleski

A spin labelling study of the hydrophobic barrier in liposomes

A fundamental property of biological membrane is to act as a barrierto permation of polar molecules. This barrier effect is largely due to a hydrophobicity of a phospholipid bilayer, determined by the extent of water penetration into the nmembrane. In order to study the depth of water penetration into liposomes, we used the electron spin resonance method (1), that takes advantage of the solvent effect on the hyperfine interaction of the nitroxide spin labels. A fatty acids with the paramagnetic nitroxide group of the spin label covalently attached at the various positions along the fatty acid chain was introduced into multilamellar liposomes. The hydrophobic barrier for water molecules, as well as, for small molecules such as glycine, was studied as a function of the liposome composition and size.

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