engleski

Insight into mechanism of cell adhesion and spreading at the charged interface

Adhesion and spreading of algal cell at the charged interface causes double layer charge displacement which is measured as a well defined amperometric signal in millisecond time scale. The reaction kinetics model enables calculation of kinetic parameters of the adhesion process from the signal, enabling to decouple and identify temporal evolution of the individual states. We study kinetics of adhesion and spreading of algal cell and its plasma membrane vesicle at the charged interface. The simple system of isolated plasma membrane vesicle without internal content is developed and characterized by atomic force microscopy. We demonstrate validity and pertinence of the reaction kinetics model in the wide range of surface charge densities. Adhesion kinetics of algal cell is slower than its plasma membrane vesicle. The model enables to associate individual states with various features of amperometric signal. At the time of current amplitude, ruptured state predominates and cell contact area is larger than contact area of the plasma membrane vesicle which contributes about one quarter to the cell contact area. These results suggest that the major structural disruption of the cell membrane, collapse of cytoskeleton and leakage of intracellular material could appear close to the time of current amplitude. Further, kinetics of spreading of organic film at the interface to its maximal extent is being considered as the rate determining step, which could be consequence of the attenuated effect of potential at the modified interface, stronger intermolecular interactions and reorganization of molecules in the film. Our findings offer insight into the mechanism of algal cell adhesion and spreading at the charged interfaces, relevant for the electroporation based studies

amperometry; cell adhesion; electrified interface; mathematical model

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