engleski

A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011-2014

Molecular epidemiology of human parainfluenza viruses type 1 (HPIV1) was investigated. Samples were collected from patients hospitalized in Croatia during the three consecutive epidemic seasons (2011- 2014). Results indicated co-circulation of two major genetic clusters of HPIV1. Samples from current study refer to clades II and III in a phylogenetic tree of hemagglutinin- neuraminidase (HN) gene. Additional phylogenetic trees of fusion (F) and phosphoprotein (P) gene confirmed the observed samples topology. Analysis of nucleotide diversity of entire P, F and HN genes demonstrated similar value ; 0.0255, 0.0236, and 0.0237, respectively. However, amino acid diversity showed F protein to be the most conserved, while P protein was the most tolerant to mutations. Potential N- and O- glycosylation sites suggested that HPIV1 HN protein is abundantly glycosylated, and a specific N-glycosylation pattern could distinguish between clades II and III. Analysis of potential O-glycosylation sites in F protein indicated that samples from this study have a two O-potential glycosylation sites while publicly available sequences have five potential sites. This study provides data on molecular characterization and epidemic pattern of HPIV1 in Croatia.

HPIV1 ; molecular epidemiology ; haemagglutinin-neuraminidase ; fusion protein ; phosphoprotein

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