engleski

Isolation of anthocyanins from grape pomace by natural deep eutectic solvents and valorization of their biological activity

Natural deep eutectic solvents (NADES) are a new generation of environmentally friendly solvents with possible applications in various industrial felds. NADES are mixtures of two or three cheap and readily available components, such as nontoxic quaternary ammonium salts (e.g., cholinium chloride) and naturally- derived uncharged hydrogen-bond donor (e.g., vitamins, amines, sugars, alcohols and carboxylic acids). Those solvents are commonly composed of cellular metabolites in specifc molar ratios, including water in some cases, and are characterized by strong intermolecular interactions (eg. hydrogen and ionic bonds). Furthermore, eutectic solvents have unique physical and chemical characteristics, and with regard to the possibility of designing their structures, it is possible to rationally design them for specifc purposes. One of the application areas of NADES is the extraction of bioactive compounds from plant materials. NADES have been reported as extraction agents for phenolic compounds due to their ability to form hydrogen bonds between the phenolic compounds and DES molecules. Based on the above mentioned, the aim of our research was extraction of anthocyanins from grape pomace by using NADES and its isolation while preserving their biological activity. Grape pomace extracts were prepared with fve different NADES in an ultrasonic bath. Anthocyanins from extracts were isolated by macroporous resin Amberlite XAD-16 and eluted with ethanol. Ethanol eluents were analysed and total content of anthocyanins and its antioxidant capacity was determined. Biological activity of anthocyanins in ethanol eluents was valorised in vitro by testing their impact on three cell lines (HeLa, MCF-7, and HEK293T). The developed procedure for isolation of anthocyanins based on extraction with NADES in combination with application of macroporous resin could be good alternative and environmental method for the extraction and enrichment of anthocyanins from grape pomace.

anthocyanins ; deep eutectic solvents ; grape pomace ; human cell lines ; in vitro biological activity ; macroporous resin

nije evidentirano