engleski

Cytotoxic and prooxidative effect of ginger extract on human laryngeal and lung carcinoma cell lines

Nowadays, cancer has become a leading cause of fatal diseases worldwide. Recently, the emphasis is dedicated on investigation of natural, alternative remedies in treatment of cancer cell lines, where plant extracts, due to their rich bioactive composition and high biological activity, may play big role. In this study, biological effect of aqueous ginger (Zingiber officinale) extract (GE) on the human laryngeal cancer cell line (HEp2) and lung carcinoma cells (A549) was evaluated. Cytotoxic, prooxidative effect and lipid peroxidation were examined in cancer cell lines exposed to different concentrations of GE for 2 hours. Cell survival was determined by Neutral red test, while 2’, 7’-dichlorofluorescein- diacetate (DCFH-DA) method was used for determination of free radicals. Lipid peroxidation was evaluated by measuring concentration of generated malondialdehydes. GE showed significant (p<0.05) decrease in cell survival for HEp2 and A549 in the highest concentration of 10 mg/mL, while lower concentrations (5 mg/mL and 1 mg/mL) did not show cytotoxic effect. GE did not exhibit prooxidative effect on both cell lines, where by increasing the extract concentration free radical induction was decreased. Moreover, GE did not reveal effect on cell lipids in A549 line, while in HEp2 cell line GE in concentration of 5 mg/mL caused lipid peroxidation. Obtained results confirmed markable health benefits of GE and justified its wide application in various pharmaceutical and food products.

Cytotoxicity ; Ginger ; Laryngeal and lung carcinoma cell lines ; Lipid peroxidation ; Prooxidative effect

nije evidentirano