engleski

In vitro testing of bioactive compounds from natural sources

Natural products such as fruits, vegetables and herbs contain different bioactive substances which vary widely in chemical structure and function. Beneficial effects of isolated bioactive compounds on human health are associated with anti-tumor, anti-allergic, antibiotic, antioxidant and other properties. Protective effects of bioactive compounds toward different types of tumors are of huge importance since the cancer is one of the leading causes of morbidity and mortality worldwide. Therefore, biologically active substances from plants are being investigated as a potential source of new drugs, wherein in vitro assays using different cell lines are frequently used. Cell lines represent a useful alternative approach in toxicological studies, from general toxicity to ecotoxicity. In addition, human cell lines (normal and tumor) are often used in testing of bioactive compounds from different plant sources. In this study, we will present results of biological activity of subcritical water extracts from different plants toward tumor cell lines (HeLa, HepG2 and MCF-7) assessed by commercial CellTiter 96® Cell Proliferation Assay (Promega, USA). Obtained results gained by in vitro tests could serve as guidelines for further in vivo studies of tested samples.

bioactive compounds, cell lines, antiproliferative activity

Rad je sufinanciran iz sredstava bilateralnog projekta Hrvatska/Srbija "Biološki potencijal subkritičnih vodenih ekstrakata iz biljnog otpada" (2016.-2017.)