engleski

Lipid composition of bovine follicular fluid during folliculogenesis

Lipids are an important component of the follicular fluid (FF) as a cellular energy source and also have important biological functions in cell membrane biogenesis and signalling. Fatty acids (FAs) in FF are present in esterifed form as triacylglycerol (TAG), cholesterol esters and phospholipids (PHL), and as nonesterifed FAs (NEFAs). The aim of the study was to determine the lipid composition of bovine FF during folliculogenesis. Bovine ovaries were collected at a slaughterhouse, and follicular fluid samples were obtained from follicles of three different sizes (<5 mm N=19 ; >5 mm N=22 ; and >10 mm N=23). Concentrations of TAG, PHL, total cholesterol (TC), low- density lipoprotein cholesterol (LDL- C), high- density lipoprotein cholesterol (HDL- C), NEFA and FAs in FF were analysed. The saturated fatty acids were dominant in bovine FF and the most common was myristic acid (C14:0). Polyunsaturated fatty acids were the second most represented, with linoleic acid (C18:2) in the highest percentage. The least represented were monounsaturated fatty acids (MUFA), with oleic acid (C18:1c9) as the most common. The percentage of C14:1, C15, C18:1c9, C20:5n-3, C22:5, MUFA, and concentrations of LDL-C, TAG and NEFA were signifcantly higher in follicles <5 mm compared to follicles >10 mm. The results also show the signifcantly lower percentage of C18:0, the ratio of arachidonic acid/eicosapentaenoic acid (C20:4n-6/C22:5n-3) and TC and PHL concentrations in follicles sized < 5 mm compared to follicles more than 10 mm in diameter. Our data show that differences in the lipid composition of FF are associated with the stage of follicle growth and development.

bovine folicular fluid , lipid composition , folliculogenesis

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