Anticancer effects of natural products from animal and plant origin (CROSBI ID 666766)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gajski, Goran ; Madunić, Josip ; Vrhovac Madunić, Ivana ; Čimbora- Zovko, Tamara ; Rak, Sanjica ; Breljak, Davorka ; Osmak, Maja ; Garaj-Vrhovac, Vera
engleski
Anticancer effects of natural products from animal and plant origin
For last couple of decades, natural products have served us well in combating different types of cancer. The main sources of these useful compounds are from both animal and plant origin. Here we will present anticancer ability of bee venom (BV) and apigenin (API) towards different types of cancer cells in vitro. BV from honey bees is a complex mixture of a variety of different active peptides while API is a natural flavonoid found in several dietary plant foods. Anticancer effect of whole BV was tested in human cervical carcinoma HeLa cells and their drug- resistant HeLa CK subline while anticancer effect of API was tested in human breast cancer MCF-7 and MDA MB-231 cells. Cytotoxicity of both compounds towards cancer cells was evaluated by MTT assay whereas type of cell death was analysed by differential staining using acridine orange/ethidium bromide and was further verified by Western blot analysis. BV displayed dose- dependent cytotoxicity against both cell lines tested with drug-resistant HeLa CK cells being more sensitive to BV than their parental cell lines. Similarly, API inhibited the growth of both cell lines in a dose-dependent manner with MCF- 7 cells being more sensitive. Treatment with BV induced a necrotic type of cell death, as shown by characteristic morphological features, fast staining with ethidium bromide and a lack of cleavage of apoptotic marker poly (ADP-ribose) polymerase (PARP) on Western blot. On the contrary, cell treated with API showed apoptosis as a dominant type of cell death in both cell lines which was further verified by Western blot analysis detecting cleaved PARP. In view of accumulating evidence on anti- proliferative and pro-cell death activity, both tested compounds could be used in the development of future anticancer drugs. Undoubtedly, therapeutic applications of BV and API are promising, however further in vitro and in vivo studies are warranted to resolve precise mechanisms responsible for their anticancer properties
bee venom ; apigenin ; human cervical carcinoma cells ; human breast cancer cells ; anticancer effects
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Podaci o prilogu
S118-S118.
2017.
objavljeno
10.15419/bmrat.v4is.316
Podaci o matičnoj publikaciji
Biomedical Research and Therapy, 4(S) ; Book of Abstracts of The 3rd International Conference CRRM 2017 - Innovations in Cancer Research and Regenerative Medicine, Ho Chi Minh, Vietnam
Ho Chi Minh City: Biomedical Research and Therapy
2198-4093
2198-4093
Podaci o skupu
The 3rd International Conference CRRM 2017 - Innovations in Cancer Research and Regenerative Medicine
poster
10.09.2017-13.09.2017
Ho-Ši-Min, Vijetnam
Povezanost rada
Biologija