engleski

Electrospinning of PCL/CEFUROXIM® fibrous carriers on 3D printed collectors

Tissue engineering provides great opportunities in the development of artificial organs, especially biological regenerative materials, such as cartilage, bone and skin. Designing the appropriate biodegradable and biocompatible materials and investigation of their biochemical and physical properties as well as cell growth factors, makes it possible to generate substitutes that can restore or substantially improve the function of damaged tissues. In this paper, the effect of different topographies of electrospun Polycaprolactone fibrous carrier on the incorporation and cell growth was investigated. Polymer carriers with different structure and porosity, have been prepared by electrospinning of PCL/CEFUROXIM® (CFU) solution at the 3D printed collectors. Polymer solution was prepared with different concentrations of antibiotic CFU, 15 and 20 %wt., which is used for local eye infection treatment. Fibrous morphology was followed by scanning electron microscopy (SEM), while the incorporation of cells in the polymer carrier was identified by Fourier transformation infrared spectroscopy (ATR-FTIR). Melting (Tm) and crystallization (Tc) temperatures of PCL/CFU as well as degree of crystallinity were determined by differential scanning calorimetry (DSC), while thermal stability of material was obtained by thermogravimetry (TG). Results of PCL/CFU carrier microstructure were correlated with transformation temperatures and antibiotic concentrations as well as cell growth.

Electrospining, PCL, fibrous carriers, cells

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