engleski

Usporedba parametara vezanja AFM1 iz mlijeka pomoću bakterija mliječne kiseline

AFM1 is an AFB1 derivate formed in the liver and is excreted in milk by the mammalian milk glands. Therefore, concerns about mycotoxins entering human food chain indirectly are well justified. In order to reduce the mycotoxins ratio, growth of molds should be reduced and that can be accomplished with good manufacturing practice (GMP). GMP alone is not sufficient to eliminate, inactivate and prevent fungal growth and toxin production so various physical and chemical methods are used. Lactic acid bacteria (LAB) are commonly used in feed production, in fermented product processes and are also known to extend the shelf-life of the product. Owing the ability of certain LAB strains to bind and neutralize mycotoxins, LAB are suggested as a potential novel biological method to reduce the toxicity of mycotoxins or prevent their absorption into the human body. Therefore, this study was carried out to investigate and compare the ability of viable, heat treated and lyophilized LAB cells to bind AFM1 in artificially contaminated milk. Depending on the used strain, incubation time and treatment of the cells, binding affinity of AFM1 by LAB cells was 23, 73-94, 49 %. Heat treated LAB cells showed highest ability (32- 94, 49 %) to bind AFM1, while lyophilized LAB cells showed lowest percentages of AFM1 binding. Obtained results indicate the importance of cellular treatment as they disturb the cell wall structure and consequently affect the ability to bind mycotoxins.

aflatoxin M1, lactic acid bacteria, viable cells, dead cells, freeze-drying

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