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Ancient DNA old as city of Split

Ancient DNA is an important tool for diverse disciplines, such as anthropology and archeology. Individual archeological site has been found occasionally, at the northern part of Split city, outside of the Diocletians palace. Extended male skeleton, age 30-40, lying on the back was found in the pit together with ceramics from later Roman period. On the right hand he had a bracelet, made of copper, with open ends. The expected maximum stature based on the length of the long bone was 167 cm (Trotter, Gleser). Estimated time since death was approximately 1760&plusmn ; 80 years (by 14 C method). DNA was succesfully extracted by the AFDIL modified method, based on standard proteinase K-phenol extraction, followed by purification with microconcentrators (AlonsoA. et al. DNATyping from Skeletal Remains: Evaluation of Multiplex and Megaplex STR Systems on DNAIsolated from Bone and Teeth Samples. Croatian Medical Journal 2001 ; 42:260-266). We simultaneously amplified nine human short tandem repeats (STR) systems and the amelogenin locus, employing AmpFlSTR Profiler. According to our experience, the high quality of extracted DNA as well as an extremely successful DNA amplification could be due to characteristics found in Split area (clay ground).

ancient DNA; DNA extraction; AmpFlSTR Profiler; archeology

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