engleski

Y-chromosomal Short Tandem Repeat A Haplotypes in Southern Croatian Male Population Defined by 17 Loci

Aim. To define the Y-chromosome genetic structure in a sample from Southern Croatia male population. Methods. The blood samples were collected at the Department of Forensic Medicine and Biochemical Laboratory of University Hospital Split, between 2004-2007, from unrelated male individuals. Genomic DNA was extracted using standard procedure. Seventeen Y chromosome short tandem repeat (STR) polymorphic loci (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, GATAH4, DYS437, DYS438, DYS448) were analyzed in a sample of 166 unrelated healthy male individuals from Southern Croatia using AmpFlSTR Yfiler PCR Amplification Kit. Results. Total haplotype diversity was 0.997289, and 141 haplotypes (84.49 %) appeared as unique. Conclusions. Combination of Y chromosome 17 STR loci presents a powerful tool for individual identification and parentage analysis in the Southern Croatian male population. Not a single haplotype that appeares more then once in Western Croatian population (17 out of 101) matches any Southern Croatian haplotype observed in more than one individual (25 out of 166).

forensic science; DNA typing; STR; population genetics; Southern Croatia; AmpFl STR Yfiler

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