engleski

Family-based analysis of tumor necrosis factor and lymphotoxin alpha tag polymorphisms and type 1 diabetes in the population of South Croatia.

Type 1 diabetes is an autoimmune disease characterised by destruction of pancreatic beta cells. It is influenced by environmental and genetic factors. TNF and LTA are cytokines with a wide range of inflammatory and immunomodulatory activities possibly related with type 1 diabetes. The aim of the present study was to evaluate the association of 11 TNF/LTA tag polymorphisms in 160 type 1 diabetes trio families from South Croatia. Investigated tag polymorphisms were designed to capture the majority (62%) of common variation in TNF/LTA gene region, based on the HapMap database. We observed overtransmision of allels from parents to affected child at four variants: rs909253, allele C, p=1.2x10-4, rs1041981, allele A, p=1.1x10-4, rs1800629 (G-308A), allele A, p=1.2x10-4 and rs361525 (G-238A), allele G, p=8.2x10-3. We also identified overtransmission of the rs1800629(G-308A)-rs361525 (G-238A) G-A haplotype, p=2.384x10-5. The present study found an association of TNF/LTA gene region with type 1 diabetes. A careful assessment of TNF/LTA variants adjusted for linkage disequilibrium with HLA loci is needed to further clarify the role of these genes in type 1 diabetes susceptibility.

Type 1 diabetes; Tumor necrosis factor; Lymphotoxin alpha; TDT; Tag polymorphism

nije evidentirano